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SRX392727: GSM1290067: SS020812 R1.0 co-culture [Shewanella]; Shewanella sp. W3-18-1; RNA-Seq
6 ABI_SOLID (AB 5500xl Genetic Analyzer) runs: 157.3M spots, 7.9G bases, 5.6Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: INTERACTIONS BETWEEN CYANOBACTERIA AND HETEROTROPHS UNDER DIFFERENT TROPHIC CONDITIONS
show Abstracthide Abstract
Application of genome-scale 'omics approaches to dissect subcellular pathways and regulatory networks governing interspecies interactions is dependent, at least initially, on the availability of model systems with well-annotated genomes and tractable genetics. Overall design: We employed controlled cultivation and next-generation sequencing technology to identify transcriptional responses of euryhaline unicellular cyanobacterium Synechococcus sp. PCC 7002 and a marine facultative aerobe Shewanella putrefaciens W3-18-1 to investigate the effect of C sources and C flux directions on the interactions between these organisms.
Sample: SS020812 R1.0 co-culture [Shewanella]
SAMN02444863 • SRS515717 • All experiments • All runs
Library:
Instrument: AB 5500xl Genetic Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted using Trizol following by DNaseI treatment using Qiagen RNAseay mini column Template cDNA was prepared using the Applied Biosystems SOLiD® Total RNA-Seq Kit (Life Technologies, Carlsbad, CA) according to manufacturer's protocol. Sequencing was carried using the SOLiDTM 5500XL protocol (Life Technologies).
Experiment attributes:
GEO Accession: GSM1290067
Links:
External link:
Runs: 6 runs, 157.3M spots, 7.9G bases, 5.6Gb
Run# of Spots# of BasesSizePublished
SRR105083725,703,9941.3G959.1Mb2014-01-08
SRR110466126,038,0161.3G973.2Mb2014-01-08
SRR110466225,454,7681.3G941.7Mb2014-01-08
SRR110466326,556,6661.3G976.3Mb2014-01-08
SRR110466426,685,8741.3G969.7Mb2014-01-08
SRR110466526,856,0341.3G964.7Mb2014-01-08

ID:
563582

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